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Antimicrobial Resistance in Salmonella Serovars Isolated From an Egg-Producing Region in Brazil Rev. Bras. Ciênc. Avic.
Benevides,VP; Rubio,MS; Alves,LBR; Barbosa,FO; Souza,AIS; Almeida,AM; Casas,MRT; Guastalli,EAL; Soares,NM; Berchieri Jr,A.
ABSTRACT Fowl paratyphoid infections are caused by different Salmonella serovars that can affect a wide range of hosts. Due to its complex epidemiology, Salmonella serovar identification is crucial for the development and implementation of monitoring and control programs in poultry farms. Moreover, the characterization of the antimicrobial resistance profiles of Salmonella strains isolated from livestock is relevant to public health because they are a common causative agent of foodborne diseases. The objective of this study was to investigate the presence of Salmonella spp. and to identify the antimicrobial resistance profiles of strains isolated in the midwestern region of São Paulo state, which accounts for the highest production of table eggs in Brazil....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial susceptibility; Foodborne disease; Salmonellosis; Multi-drug resistance; Public health.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200319
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Duplex Real-Time PCR Using Sybr Green I for Quantification and Differential Diagnosis between Salmonella Enteritidis and Salmonella Typhimurium Rev. Bras. Ciênc. Avic.
Rubio,MS; Penha Fº,RAC; Almeida,AM; Barbosa,FO; Berchieri Jr,A.
ABSTRACT The incidence of foodborne diseases caused by the genus Salmonella spp. in industrialized countries is often high in epidemiological surveys. Obtaining a rapid diagnostic test for identification of bacteria is crucial in order to rapidly implement control measures to contain bacterial spread, to reduce losses in animal production and to avoid risks from food-borne infections to human health. The aim of this study was to standardize duplex real-time PCR using SYBr Green I for differential and quantitative diagnosis of S. Typhimurium and S. Enteritidis. According to the experiment, the melting temperature of 85°C was observed for a 206bp amplified product when S. Enteritidis DNA was added to the reaction. S. Typhimurium DNA showed that the melting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Identification; Melting curve; Non-typhoidal salmonellosis; Standard curve.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100317
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Performance and Serum Biochemical Profile of Broiler Chickens Supplemented with Piper Cubeba Ethanolic Extract Rev. Bras. Ciênc. Avic.
Rubio,MS; Laurentiz,AC; Sobrane Fº,ST; Mello,ES; Filardi,RS; Silva,MLA; Laurentiz,RS.
ABSTRACT Piper cubeba is an Asian pepper used in popular medicine for its known antimicrobial, antiparasitic, and anti-inflammatory actions. The study evaluated the effects of dietary inclusion of Piper cubeba ethanolic extract (PE) as a replacement for anantibiotic growth promoter (AGP) on the performance and biochemical serum profile of 1- to 21-d-old broilers (Cobb®). Two hundred one-d-old broilers were housed in experimental battery cages and distributed in a completely randomized design. The following treatments were applied: negative control (NC) - basal diet; positive control (PC) - basal diet with addition of AGP; and the basal diet with inclusion of 0.17%, 0.34%, and 0.52% of PE (NCE1, NCE2, and NCE3, respectively). Growth performance, biochemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Additives; Antibiotic growth promoters; Biochemical serum profile; Pepper.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100310
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